Locomotion is important to many complex behaviors such as foraging, mating and evading predators, and is closely related to metabolism. The gut microbiome is known to modulate various aspects of host physiology, including behavior and metabolism. Studies in mice have previously implicated microbial involvement in locomotion; however, potential pathways linking bacteria to motor behaviors have been relatively unclear. Due to the similarities in locomotion between arthropods and mammals, we set out to examine if the microbiota modulates locomotion in flies and determine the underlying mechanisms for this regulation.